return to POCT index

return to index page

Test Directory - HbA1c (POCT)

POCT Equipment

Afinion AS100 Analyzer

Clinical Indications

HbA1c is a form haemoglobin which has been non-enzymatically glycated. The amount of HbA1c present reflects blood glucose levels during the life of the red cells (normally 120 days). Production of rapid quantitative HbA1c results may be used in monitoring glycaemic control in known diabetics and should not be used for diagnosis since there is a significant overlap between diabetic and non-diabetic subjects.

Sample Type

Capillary whole blood (e.g. finger-prick) or venous whole blood with EDTA, heparin, citrate or sodium fluoride anti-coagulant.

Sample Container

None required for finger-prick samples.

Sample Volume

1.5 uL

Sample Handling

Finger-prick samples must be analysed immediately.

Anticoagulated venous samples can be stored in the fridge for up to 10 days before analysis, and can be sampled cold.

Special Precautions

Do not excessively squeeze the patientís finger which dilutes the sample with tissue fluid.

Turnaround Time

A result is produced within 3 minutes and 15 seconds

Reference Range

Please see HbA1c Conversion Table


Coagulated or haemolysed samples cannot be used.

No result will be reported if the haemoglobin value is below 6 g/dL or above 20 g/dL.

Time Limit for Additional Tests

Finger-prick samples can only be used once.

If the test result is questionable or if clinical signs and symptoms appear inconsistent with the test result, re-test with a fresh sample or confirm the result by sending a sample to the laboratory.


The test should only be carried out by a trained member of staff.

Hospital Procedure (SOP)

Standard Operating Procedure for the Measurement of Glycated Haemoglobin using the Afinion AS100 HbA1c Analyser

Last edited 16/04/14