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PTD - 161

Test Directory -  Alpha-1 Antitrypsin Phenotyping

Clinical Indications

Further investigation of low serum alpha-1 antitrypsin levels.  

Request Form

Combined Pathology Blood form (Yellow/Black)


Analysed by Protein Reference Unit, Sheffield if specific criteria met.

Specific Criteria

Total alpha-1 antitrypsin measured and referred for phenotyping if level is less than 1.2 g/l and alpha-1 antitrypsin has been specifically requested. If low alpha-1 antitrypsin has been picked up as an incidental finding from electrophoresis then a comment will be added to the report asking for the requesting clinician to discuss with the patient before phenotyping is carried out. 
Patients with alpha-1 antitrypsin levels between 0.8 and 1.2 g/L are probably carriers and may not require further investigation.

Patient preparation None required

Turnaround Time

1 month




2 ml


Yellow top (SST) tube or

Paediatric orange top (lithium-heparin)

Causes for Rejection

Serum alpha-1 antitrypsin level greater than 1.2 g/L.


Information on phenotypes. A patient information leaflet is available.

A sample for genotyping (5mL pink-top EDTA) will be requested as a follow up confirmation for deficiency alleles identified by phenotyping.

Lab. Handling

Processing: Aliquot and store at 4C in separating fridge (CB39).
:  AATP and send or BPRO and save in separating freezer (CB40) at -20C, saved frozen rack.


Version 1.0 / April 2014                                                                                                           Approved by: Consultant Biochemist