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PTD - 170

Test Directory - Androstenedione



Clinical Indications

Androstenedione may be useful in investigating the cause of a high testosterone in women. Androstenedione is not as useful as 17-OH progesterone in diagnosis of congenital adrenal hyperplasia (CAH) due to 11- or 21-hydroxylase deficiency, but is helpful in management of such patients. In CAH due to 17-hydroxysteroid dehydrogenase deficiency male (46 XY) babies have female or ambiguous genitalia and at puberty, marked virilization occurs. The condition is characterised by increased androstenedione relative to that of testosterone.

Request Form

Combined Pathology Blood form (Yellow/Black)


Referred test: Analysed by Biochemistry, Southend Hospital if specific criteria met.

Specific Criteria

Management of CAH due to 11- or 21-hydroxylase deficiency. Diagnosis of 17-hydroxysteroid dehydrogenase deficiency.

Patient Preparation

It is advisable to avoid stress. For diagnosis of 17-hydroxysteroid dehydrogenase deficiency in infants, stimulation with hCG may be required to achieve serum androstenedione levels that can be measured for accurate diagnosis.

Turnaround Time

Two weeks.




1 ml


Yellow top (SST) tube or

Paediatric orange top (lithium-heparin)

Causes for Rejection

Not meeting specific criteria for analysis. Unlabelled sample.

Reference Range

Adult: Male 2.1 - 12.9 nmol/L,
Adult: Female 1.0 - 12.9 nmol/L.

Reference: Southend Clinical Biochemistry

Lab. Handling

Processing: Aliquot within 4 hours of collection and freeze at -20C in separating freezer (CB40), active frozen rack 110.
AND and send frozen in the transport box to the referral laboratory or NOAND and save in separating freezer (CB40) at -20C, saved frozen rack.

Version 1.2 / February 2015                                                                                                   Approved by: Consultant Biochemist