return to C index  

return to index page

PTD - 215

Test Directory -  Cryoproteins

Synonyms

Cryoglobulins, cold agglutinins, immune complexes.

Clinical Indications

Cryoproteins are proteins which form a gel or precipitate on cooling the serum or plasma and re-dissolve when warmed to 37'C. Cryoglobulins are immunoglobulins that exhibit this phenomenon and may be either monoclonal (25%) or mixed (with (18%) or without (50%) monoclonal components). 

Availability

On request

Specific Criteria

Cryoprotein studies are indicated in any patient showing clinical manifestations including intolerance of cold with pain in exposed area's, Raynaud's phenomenon, and skin manifestations including purpura, urticaria and ulcers.

Patient Preperation Patient's should ideally be fasting for this test

Turnaround Time

One week

Specimen

Serum and Plasma

Volume

2 ml

Container

Pre-warmed red top (plain) tube and purple top (EDTA) tubes:




Additional yellow top (SST) tube at room temperature:


Patients must be bled at Basildon Hospital. GP and outpatient requests can attend Monday to Friday (9.00am to 4.30pm only) without having to book an appointment. For inpatients please contact Clinical Biochemistry ext 4991 to book a collection.

1. Take first blood sample into pre-warmed red top (serum) tube and
    immediately place in flask containing a 37C water tube.

2. Take second blood sample into pre-warmed purple top (EDTA) tube and
    immediately place in flask containing 37C water. Replace the flask lid.
    Note: The laboratory will, in this instance only, accept unlabelled samples
    in the flask, provided they are accompanied by the request form.) 

3. Take flask with samples and relevant request form (along with any other
    samples taken at room temperature) to Clinical Biochemistry within 10
    minutes of collection.

Causes for Rejection

Samples not collected using pre-warmed (37C) collection tubes, not transported in pre-warmed flask or delay in samples reaching laboratory. 

Interpretation

Monoclonal cryoglobulins may be associated with immunocytomata and are usually an IgM protein. Mixed cryoglobulins may be associated with lymphoproliferative disorders, but the majority are idiopathic.

Lab. Handling

Test code CRYO. Upon arrival in the laboratory samples must be centrifuged at 37C for 10 minutes at 3000 rpm.

Aliquot samples as follows:

   

1.

Aliquot of serum into pre-warmed tube labelled 'serum 37C'. Transfer directly to Biochemistry incubator for storage until analysis.

2.

Aliquot of serum into tube labelled 'serum 4C'. Store at 4C in the separating fridge (CB39)

3.

Aliquot of EDTA plasma into pre-warmed tube labelled 'plasma 37C'. Transfer directly to Biochemistry incubator for storage until analysis.

4.

Aliquot of EDTA plasma into tube labelled 'plasma 4C'. Store at 4C in the separating fridge (CB39)

Separated samples must be stored for at least 72 hours before analysis can begin.


Version 1.1 / June 2015                                                                                                          Approved by: Consultant Biochemist