PTD - 23

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Test Directory - Folate

Clinical Indications


Investigation of Macrocytic anaemia

Request Form

Combined Pathology Blood form (Yellow/Black or Blue for GP's)


Serum Folate: On request.
Red Cell Folate: Refer to Consultant Haematologist

Specific Criteria

Must be on initial request and cannot be added on later.

Turnaround Time

Same day



Samples should not be taken from patients receiving therapy with high Biotin doses (>5mg/day) until at least 8 hours following last biotin administration.




5 ml


Yellow top (SST) tube or

Paediatric orange top (lithium-heparin)

Causes for Rejection

Unlabelled or incorrectly labelled or insufficient patient identifiers sample. Not meeting specific criteria for analysis. Delay in sample reaching laboratory.  Insufficient sample volume. Poor sample quality due to deterioration, haemolysis.

Reference Range

Greater than 4.6 ug/L. Reference: Roche method insert



Results should be treated on a case by case basis and should be assessed in conjunction with patient's medical history, clinical examinations and other findings

Lab. Handling   Processing: Analysed from primary tube and stored at 4C in the cold room (CB23) in Clinical Biochemistry.

Version 1.1 / July 2014                                                                                                            Approved by: Consultant Biochemist