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PTD - 243

Test Directory - Gonadotrophins (LH/FSH)


Luteinising Hormone (LH) and Follicle Stimulating Hormone (FSH)

Clinical Indications

LH and FSH are anterior pituitary hormones which regulate male and female gonadal function. Measurement of LH/FSH can be useful in suspected disorders of gonadal function.

Test Includes


Request Form

Combined Pathology Blood form (Yellow/Black)

Patient Preparation

None required. If patient is having menstrual cycles, collect between days 1 to 5 of cycle.



LH and FSH are suppressed in patients taking combined oral contraceptive pill.


On request

Specific Criteria

Investigation of suspected hypothalamic, pituitary or gonadal dysfunction. Please refer to guidelines on appropriate investigations for women with secondary amenorrhoea and/or menopausal symptoms.

Turnaround Time

Same day




1 ml


Yellow top (SST) tube or

Paediatric orange top (lithium-heparin)

Causes for Rejection

Unlabelled sample

Reference Range

In women, dependent on stage of menstral cycle; in the early follicular phase, and in men, levels of LH and FSH are usually < 10 U/L.

Female (follicular): FSH 3.5 - 12.5 IU/L; LH 2.4 - 12.6 IU/L
Male: FSH 1.5 - 12.4 IU/L; LH 1.7 - 8.6 IU/L

Reference: Roche method insert


Low LH/FSH: pituitary / hypothalamic disease.

High gonadotrophins: primary gonadal failure (LH and FSH), azoospermia / ovulatory failure (FSH), polycystic ovary syndrome (LH>FSH).

Towards the menopause, FSH tends to rise before LH, although both show considerable variation. After the menopause, levels of both FSH and LH are greatly elevated (greater than 28 U/L).

Lab Handling

Processing: Analysed from primary tube and stored at 4C in the cold room (CB23)

Version 1.1 / July 2014                                                                                                            Approved by: Consultant Biochemist