PTD - 25

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Test Directory - Granulocyte Antibodies

Synonyms

White Cell Antibodies

Clinical Indications

To determine  granulocyte antibody significance in patients with severe neutropenia ( cause unknown )

Request Form

Combined Pathology Blood form (Yellow/Black or Blue for GP's) following discussion with or requested by Consultant Haematologists.

Availability

Analysed by referral laboratory if specific criteria met. (NBS Bristol)

Specific Criteria

Requested by Consultant or agreed with Consultant Haematologists.

Turnaround Time

Usually within two months

Specimen

Whole Blood and Serum

Volume

2 x 6ml EDTA & 1 x 6ml SST clot.

Container

2 x Purple top (EDTA) tube.

1 x Yellow top (SST) tube.

Collection

Samples should be bled early in the day (Monday to Thursday) and transported to laboratory immediately.

Lab. Handling

Samples should be posted to the Reference Laboratory as soon as possible to reach reference lab within 72 hours of bleeding.

Causes for Rejection

Unlabelled or inadequately / incorrectly filled in sample. Not meeting specific criteria for analysis. Delay in sample reaching laboratory. Underfilled, clotted or haemolysed samples.

Limitations

 

Postage delays combined with testing window (reason for collection criteria)

Interpretation

Testing for Granulocyte antibodies will only be undertaken if the condition falls into the following categories.

  1. Autoimmune neutropenia, caused by rare granulocyte autoantibodies. The condition in children between 6 months and 5 years, known as autoimmune neutropenia of infancy, is self limiting but may last several years. In adults, the condition may present as an isolated primary disorder or be secondary to other conditions such as rheumatoid arthritis or SLE.

  2. Neonatal neutropenia, caused by maternal antibodies present on granulocytes at birth, leading to the risk of infectious complications.

  3. Severe non haemolytic transfusion reactions.

  4. Transfusion related acute lung injury (TRALI)

  5. Neutropenia following bone marrow transplant.

  6. Drug induced antibody neutropenia. This test must be accompanied by a sample of the drug involved.

        

 


Version 1.0 / January 2013