return to C index

return to index page

PTD - 321

Test Directory -  Gut Hormone Profile

Synonyms

GIT, gastrointestinal tumour

Test includes

Gastrin, Vaso Intestinal Polypeptide (VIP), pancreatic polypeptide (PP), glucagon, neurotensin, somatostatin, chromagranin A & B.

Clinical Indications

Diagnosis of patients with symptoms suggestive of a secreting neuro-endocrine tumour, and screening of patients with multiple endocrine neoplasia type 1.

As an alternative marker, Chromogranin A is produced by a variety of normal and malignant neuroendocrine and sympathetic neuronal cells and may serve as a clinically useful marker for hormone-negative endocrine neoplasia.
Elevated levels of Chromogranin A are seen in neuroendocrine tumours, including phaeochromocytoma, medullary carcinoma of thyroid, pancreatic islet cell adenoma, carcinoid tumour, anterior pituitary adenoma, carotid body tumour, small cell carcinoma of the lung and neuroblastoma. 

Minor increases are seen in essential hypertension. Chromogranin A concentrations rise markedly in patients with renal failure.

Request Form

Combined Pathology Blood form (Yellow/Black)

Availability

Referred test: Analysed by Clinical Biochemistry, Charing Cross Hospital, if specific criteria met.

Specific Criteria

Investigation of suspected neuroendocrine tumour, if specifically requested by a Consultant. 

Patient Preparation

Patient should be fasting. H2 antagonists should be stopped for 72 hours, and omeprazole for 2 weeks. Patients must attend Basildon phlebotomy for sample collection.

Turnaround Time

1 month

Specimen

Large purple top (EDTA) tube
Samples must be transported to laboratory immediately.
Yellow top (SST) tubes should not be used.

Volume

2 ml

Causes for Rejection

Not meeting specific criteria for analysis.

Reference Range

Gut hormone profile reference ranges:
VIP: < 30 pmol/L

PP: < 300 pmol/L
Gastrin: < 40 pmol/L
Glucagon: < 50 pmol/L
Somatostatin: < 150 pmol/L
Neurotensin: < 100 pmol/L

Lab. Handling

Processing: Centrifuge and aliquot within 15 minutes of venepuncture into 2 tubes and freeze immediately at -20C in the separating freezer (CB40), active frozen rack 110.
Referral: GIT and send or BPRO and save in separating freezer (CB40), at -20C in the saved frozen rack.


Version 1.0 / August 2015                                                                                                       Approved by: Consultant Biochemist