PTD - 31

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Test Directory - HLA B27 / TISSUE TYPING


Clinical Indications

For use in association between HLA B27 and disease eg ankylosing spondylitis.

Request Form

Combined Pathology Blood form (Yellow/Black or Blue for GP's)


Analysed by referral laboratory (Southend Hospital)

Specific Criteria


Turnaround Time

Usually within 7-10 days. (Longer if referred due to equivocal results)


Whole Blood


1 x 6ml EDTA


Purple top (EDTA) tube.


Monday  - Thursday samples can be taken all day.
Friday - Samples bled at Basildon until 12:00
Saturday & Sunday - Do not bleed.
Samples should be transported to laboratory immediately.

Lab. Handling

Samples must be tested within 48 hours of bleeding. Friday bled samples must have arrived for 13:00 dispatch.

Causes for Rejection

Unlabelled sample. Not meeting specific criteria for analysis. Delay in sample reaching laboratory. Underfilled / insufficient sample. Poor sample quality due to deterioration, haemolysis or clot. 


  Testing window period and samples arriving at referral laboratory (hence collection times)


The presence of  HLA B27 has been shown to be associated with the condition ankylosing spondylitis. Testing for the HLA B27 antigen is a good diagnostic aid in determining the connection between a positive test result and a persistent back pain.



Version 1.0 / January 2013