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Test Directory -  Neisseria gonorrhoeae detection by PCR

Test Includes

Detection of Neisseria gonorrhoeae by strand displacement amplification assay

Request Form 

Microbiology form (Green)

Availability

On request during normal working hours

Turnaround Time

Results will be available within 3 working days of receipt of sample in the laboratory

Specimen

Cervical and/or urethral swabs from female patients, urethral swabs from male patients. Urine samples can also be used for Neisseria gonorrhoeae  detection in male patients (see collection below). The assay is NOT validated for eye samples.

Container

Special swabs together with a transportation tube are available for Neisseria gonorrhoeae detection.  The swabs are available from the Virology Laboratory (ext 3536) and can be stored on the ward for several months at room temperature.  An expiry date is printed on the package - please check EVERY swab before using.

Urine samples for detection of Neisseria gonorrhoeae must be collected into a plain, white capped, universal bottle and transferred to a UPT tube immediately after collection as below.

Collection

Urine samples: The patient should not have urinated for at least 1 hour prior to specimen collection. The first 15 to 60mL of specimen should be collected into a sterile preservative-free collection container. Using the pipette supplied in the specimen collection kit, transfer urine into the UPT tube until the sample level is between the black lines on the fill window and send to the laboratory as soon as possible.

Endocervical specimens: Use the large cleaning swab to remove excess mucous form the cervical os. Insert the collection swab into the cervical canal and rotate it for 15 to 30 seconds. Withdraw the swab carefully avoiding contamination from the vaginal mucosa. Replace the swab in the transport tube, label and send to the laboratory as soon as possible.

Urethral specimens: Insert the swab 2 to 4cm into the urethra and rotate it for 3 to 5 seconds. Replace the swab in the transport tube, label and send to the laboratory as soon as possible.

Lab. Handling

Samples will be processed as soon as possible following receipt.

Causes for Rejection

Incorrectly and/or Insufficiently labelled sample
Delay in sample reaching laboratory
Incorrect swab type or urine sample container received
Out of date specimen collection swab/UPT.

 

Version 1.0 / November 2013

Last edited 16/04/14