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PTD - 271

Test Directory -  Oestradiol


Estradiol, E2

Clinical Indications

Investigation of amenorrhoea. Investigation of gynaecomastia in men. Monitoring of patients with HRT (oestradiol) implants. There is little value in measuring oestradiol for monitoring of oral HRT or patches other than testing for non-compliance or non-absorption.

Oestradiol levels are of no value in investigation of menopause since levels may remain normal for several months following menopause (please refer to protocol on investigation of menopause).

Request Form 

Combined Pathology Blood form (Yellow/Black or Blue for GP's).


Referred test: Analysed by Clinical Biochemistry, Southend Hospital, if specific criteria met.

Specific Criteria


Patient Preparation

None required.

Turnaround Time

Same day




1 mL


Yellow top (SST) tube or

Paediatric orange top (lithium-heparin)

Causes for Rejection

Unlabelled sample.

Reference ranges


Patient group

Oestradiol (pmol/L)

Boys (1 - 10 years)

Less than 73.4

Girls (1 - 10 years)

22 - 99.1


28 - 156

Women - Follicular phase

46 - 607

Women - Ovulation phase

315 - 1,828

Women - Luteal phase

161 - 774

Women - Post menopause

Less than 201

Pregnancy (1st trimester)

789 - >15,781

Reference: Roche method insert

Lab Handling


Processing: Aliquot (MPA) and store at 4C in separating fridge (CB39).

Referral: SOES and send or BPRO and save in separating freezer (CB40) at -20C, saved frozen rack.


Version 1.1 / June 2015                                                                                                          Approved by: Consultant Biochemist