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PTD - 306

Test Directory - hsTroponin T


cTnT, high sensitivity Troponin T

Clinical Indications

Troponin T is a regulatory protein that helps heart muscle contract and is released into blood when cell damage occurs. Its use is in further investigation and risk stratification of patients with chest pain of undiagnosed origin.

Request Form

Combined Pathology Blood form (Yellow/Black)


24/7. Requests will be processed as soon as possible.

Specific Criteria

Please ensure that there is reasonable suspicion of acute myocardial ischaemia (clinical symptoms, ECG changes, history) before requesting hsTroponin since false positive results due to chronic elevations in other diseases such as chest infection, renal impairment are common.

Patient Preparation

Samples should not be taken from patients receiving therapy with high
biotin doses (i.e. > 5 mg/day) until at least 8 hours following the last biotin


Haemolysis causes falsely low results.

Turnaround Time

Same Day (within 2 hours)




2 ml


Yellow top (SST) tube. Lithium heparin (green top) samples are also acceptable.

Causes for Rejection

Not meeting specific criteria for analysis. Haemolysed sample.

Reference Range

A cut-off level of 14 ng/L (99th centile) is used. Providing the sample has been taken at least 6 hours after onset of symptoms a level below 14 ng/L rules out significant cardiac damage. Levels above 14 ng/L may be due to either a chronically raised level (e.g. in renal failure patients) or an acute rise seen in acute coronary syndrome. In these patients a second Troponin T level after 6 hours may be indicated.

Lab Handling


Processing: Analysed from primary tube and stored at 4C in the cold room (CB23)


Version 1.1 / February 2015                                                                                                    Approved by: Consultant Biochemist